Molecular Methods for Identification and Genotyping of BK Virus

General properties of human polyomavirus BK (BKV) such as its clinical significance, propagation, and serological assays are described in Chapter 3. In this chapter, two molecular methods for detection of a subtype-specific sequence of BKV are described. Both methods were developed based on polymerase chain reaction (PCR) amplification and subsequent identification of BKV subtypes by either sequencing or restriction enzyme analysis.