Freeze-Fracture for Immunofluorescence of Embryos
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<center> <u>Materials:</u></center>
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disposable scapel |
#15 (round tip) Feather brand |
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subbed slides |
0.1 % poly-L-lysine (MW>300,000) |
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0.2 % gelatin |
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0.01 % chrome alum |
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(dip slides in solution and dry overnight) |
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Whatmann paper |
for ease cut into 1 inch square pieces |
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compiled by Chad Rappleye
Aroian Lab Protocols
