Freeze-Fracture for Immunofluorescence of Embryos
互联网
697
<center> <u>Materials:</u></center>
|
disposable scapel |
#15 (round tip) Feather brand |
|
|
|
|
subbed slides |
0.1 % poly-L-lysine (MW>300,000) |
|
|
0.2 % gelatin |
|
|
0.01 % chrome alum |
|
|
(dip slides in solution and dry overnight) |
|
|
|
|
Whatmann paper |
for ease cut into 1 inch square pieces |
|
|
|
compiled by Chad Rappleye
Aroian Lab Protocols
