• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Generation of Lung Epithelial-Like Tissue from hESC by AirLiquid Interface Culture

        互联网

        435
        Human embryonic stem cells (hESCs) have the capacity to differentiate in vivo and in vitro into cells from all three germ lineages. The in vitro generation of lung cells and tissues from hESCs creates opportunities for fundamental research, drug development or cell-replacement therapy. In this chapter, we describe a reliable and simple protocol to differentiate hESCs into the major cell types of lung epithelial tissue. In this protocol, undifferentiated hESCs, grown on a porous membrane in hESC medium for 4 days, are switched to a differentiation medium and cultured for 4 days, followed by differentiation in air–liquid interface conditions for 20 days. The expression of several lung markers is analysed using quantitative real-time PCR and confirmed by immunohistochemistry. The functionality is determined through an enzyme immunoassay. For a more detailed discussion regarding the obtained cells and their gene and protein expression, refer to Van Haute et al. (Generation of lung epithelial-like tissue from human embryonic stem cells Respiratory Research10: 105, 2009).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序