• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes

        互联网

        440
        Although the polysaccharide backbone Glycosaminoglycans (GAGs) have been demonstrated to interact with a variety of proteins and such interactions are thought to be involved in the regulation of the physiological functions of these proteins (1 ). Although the polysaccharide backbone of GAGs is a linear polymer composed of alternating amino sugar and hexuronic acid residues, this simple repeat structure acquires a considerable degree of variability by extensive modifications involving sulfations and uronate epimerization (2 ). The structural variability is the basis for the wide variety of domain structures with biological activities (1 ). Investigation of the structure-function relationship of GAGs has been hindered by the difficulty in microanalyzing their complicated structure. GAG molecules are so heterogeneous that sequence analysis on unfractionated GAG chains can give only statistical structural information. Actual sequencing is possible, however, on oligosaccharide fragments, which can be obtained by chemical or enzymatic degradation of GAG chains followed by separation by means of various chromatographies. In this chapter, we describe the methods for enzymatic degradation of GAG chains and fractionation of the oligosaccharide products. Because many kinds of highly purified GAG-degrading enzymes, which are not contaminated by sulfatases, are commercially available and cleave polysaccharides with high specificities under mild conditions, enzymatic cleavage is a useful method for the preparation of GAG oligosaccharides.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序