Recovery of Proteins and Phase Components

Two-phase extraction of protein mixtures leads to one or several protein fractions, which also contain mainly one of the phase-forming polymers. To separate proteins from the phase polymer, a number of methods can be used (1 ). If proteins and polymer differ strongly in their respective molecular weights (10 times or more), they can be separated by gel chromatography or ultrafiltration (2 ,3 ). A more common way is to use ion-exchange chromatography (1 ). Other methods are those based on electrophoresis (4 ) and two-phase techniques (1 ,4 ,5 ).