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        Common File Formats

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        722
        • Abstract
        • Table of Contents
        • Figures
        • Literature Cited

        Abstract

         

        This appendix discusses a few of the file formats frequently encountered in bioinformatics. Specifically, it reviews the rules for generating FASTA files and provides guidance for interpreting NCBI descriptor lines, commonly found in FASTA files. In addition, it reviews the construction of GenBank, Phylip, MSF and Nexus files.

        Keywords: file format; FASTA; NCBI descriptor lines; GenBank; Phylip; MSF; Nexus

             
         
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library

        Table of Contents

        • FASTA Files
        • GenBank Flat Files
        • Phylip Files
        • MSF Files
        • Nexus Files
        • Converting between File Formats
        • Disclaimer
        • Figures
        • Tables
             
         
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library

        Materials

         
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library

        Figures

        •   Figure a0.1B.1 A sample FASTA file that contains the sequences for two homologous proteins, actophorin and yeast cofilin. Note that a greater‐than sign (>) designates the beginning of each entry and that each of the lines of sequence contains less than 80 characters.
          View Image
        •   Figure a0.1B.2 A sample GenBank record. Circled numbers identify the fields listed in Table .
          View Image
        •   Figure a0.1B.3 A sample PHYLIP‐formatted file. The five sequences shown are HIV‐1 and HIV‐2 gag proteins from a variety of isolates. See text for details.
          View Image
        •   Figure a0.1B.4 A sample MSF‐formatted file. The five sequences shown are HIV‐1 and HIV‐2 gag proteins from a variety of isolates. See text for details.
          View Image
        •   Figure a0.1B.5 A sample Nexus‐formatted file. The five sequences shown are HIV‐1 and HIV‐2 gag proteins from a variety of isolates. See text for details.
          View Image

        Videos

        Literature Cited

        Internet Resources
           http://iubio.bio.indiana.edu/cgi‐bin/readseq.cgi
           ReadSeq biosequence interconversion tool.
           http://www.ebi.ac.uk/clustalw
           ClustalW multiple sequence alignment interface.
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