关于寻找基因序列
丁香园论坛
1419
我的试验动物是猪，在网上查的HIF的基因不是全序列，对引物设计有影响吧，如果这样不能得到引物我该怎样办？我急用求高人指点
LOCUS AY485675 569 bp mRNA linear MAM 23-DEC-2003
DEFINITION Sus scrofa hypoxia-inducible factor 1 alpha mRNA, partial cds.
ACCESSION AY485675
VERSION AY485675.1 GI:40037189
KEYWORDS .
SOURCE Sus scrofa (pig)
ORGANISM Sus scrofa
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Cetartiodactyla; Suina; Suidae; Sus.
REFERENCE 1 (bases 1 to 569)
AUTHORS Ing,N.H., Balog,C.J. and Wolfskill,R.L.
TITLE Direct Submission
JOURNAL Submitted (21-NOV-2003) Department of Animal Science, Texas A&M
University, 410 Kleberg, College Station, TX 77843-2471, USA
FEATURES Location/Qualifiers
source 1..569
/organism="Sus scrofa"
/mol_type="mRNA"
/isolation_source="endometrium of a sow in day 13 of the
estrus cycle"
/db_xref="taxon:9823"
CDS <1..>569
/note="HIF"
/codon_start=2
/product="hypoxia-inducible factor 1 alpha"
/protein_id="AAR37392.1"
/db_xref="GI:40037190"
/translation="RSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLL
DAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHS
VFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATW
KVLPCTGHIHVYDTNNNQSQCGYKKPPMT"
ORIGIN
1 acgaagtaaa gagtctgaag ttttttatga gcttgctcat cagttgccac ttccccataa
61 tgtgagctca catcttgata aggcttctgt tatgaggctt accatcagct atttgcgtgt
121 gaggaaactt ctagatgctg gtgatttgga tattgaagat gaaatgaagg cgcagatgaa
181 ttgtttttat ttgaaagcct tggatggttt tgttatggta ctcacagatg atggtgacat
241 gatttatatt tctgataatg tgaacaaata catgggatta actcagtttg aactaactgg
301 acacagtgtg tttgatttta ctcatccgtg cgaccatgag gaaatgagag aaatgcttac
361 acacagaaat ggccttgtga aaaagggtaa agagcaaaat acacagcgga gcttttttct
421 cagaatgaag tgtaccttaa ctagcagggg gagaactatg aacataaagt ctgcaacatg
481 gaaagtactt ccgtgcacag gncatattca tgtatatgat accaacaata accagtctca
541 gtgtgggtat aagaaaccac ctatgacgt
LOCUS AY485675 569 bp mRNA linear MAM 23-DEC-2003
DEFINITION Sus scrofa hypoxia-inducible factor 1 alpha mRNA, partial cds.
ACCESSION AY485675
VERSION AY485675.1 GI:40037189
KEYWORDS .
SOURCE Sus scrofa (pig)
ORGANISM Sus scrofa
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Cetartiodactyla; Suina; Suidae; Sus.
REFERENCE 1 (bases 1 to 569)
AUTHORS Ing,N.H., Balog,C.J. and Wolfskill,R.L.
TITLE Direct Submission
JOURNAL Submitted (21-NOV-2003) Department of Animal Science, Texas A&M
University, 410 Kleberg, College Station, TX 77843-2471, USA
FEATURES Location/Qualifiers
source 1..569
/organism="Sus scrofa"
/mol_type="mRNA"
/isolation_source="endometrium of a sow in day 13 of the
estrus cycle"
/db_xref="taxon:9823"
CDS <1..>569
/note="HIF"
/codon_start=2
/product="hypoxia-inducible factor 1 alpha"
/protein_id="AAR37392.1"
/db_xref="GI:40037190"
/translation="RSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLL
DAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHS
VFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATW
KVLPCTGHIHVYDTNNNQSQCGYKKPPMT"
ORIGIN
1 acgaagtaaa gagtctgaag ttttttatga gcttgctcat cagttgccac ttccccataa
61 tgtgagctca catcttgata aggcttctgt tatgaggctt accatcagct atttgcgtgt
121 gaggaaactt ctagatgctg gtgatttgga tattgaagat gaaatgaagg cgcagatgaa
181 ttgtttttat ttgaaagcct tggatggttt tgttatggta ctcacagatg atggtgacat
241 gatttatatt tctgataatg tgaacaaata catgggatta actcagtttg aactaactgg
301 acacagtgtg tttgatttta ctcatccgtg cgaccatgag gaaatgagag aaatgcttac
361 acacagaaat ggccttgtga aaaagggtaa agagcaaaat acacagcgga gcttttttct
421 cagaatgaag tgtaccttaa ctagcagggg gagaactatg aacataaagt ctgcaacatg
481 gaaagtactt ccgtgcacag gncatattca tgtatatgat accaacaata accagtctca
541 gtgtgggtat aagaaaccac ctatgacgt
