CrystalDirect: A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films

The last years have seen a major development in automation for protein production, crystallization, and X-ray diffraction data collection, which has contributed to accelerate the pace of structure solution and to facilitate the study of ever more challenging targets through macromolecular crystallography. This has led to a considerable increase in the numbers of crystals produced and analyzed. However the process of recovering crystals from crystallization supports and mounting them in X-ray data collection pins remains a manual and delicate operation. Here we present a novel approach enabling full automation of the crystal mounting process and describe the operation of the first-automated CrystalDirect harvesting unit. Implications for crystallography applications and for the future operational integration of automated crystallization and data collection resources are discussed.