Analysis of N- and O-Glycosylation of Plant Proteins

In eukaryotic cells, secreted proteins can be subjected to numerous post-translational modifications. One of these, glycosylation, consists of the addition of sugar residues to the protein backbone, while the protein enters or travels within the secretory pathway. Glycosylation of secreted proteins can be of two types∶ N - or O -glycosylation, depending on the linkage involved between the oligosaccharide moiety and the protein backbone.