• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Genomic Mismatch Scanning for the Mapping of Genetic Traits

        互联网

        582
        Genome mismatch scanning (GMS) is a rapid method of isolating regions of identity by descent (IBD) between two related individuals (1 5 ). With the availability of simple PCR techniques, vast numbers of highly informative genomewide polymorphic markers, and more recently, radiation hybrid mapping, DNA microarrays, and gene chip technology (Research Genetics, AL), GMS is a very practical shortcut to conventional genetic linkage methods. The basic procedure (Fig. 1 ) involves the restriction enzyme digestion of each of the genomic DNA samples from two related individuals, yielding fragment sizes up to 20 kb, followed by the methylation of one genome. Hybridization of the two genomes (one fully methylated and one fully unmethylated) results in four possible DNA hybrid fragments. Through specific restriction enzyme digestions, the fully methylated and the fully unmethylated homohybrids (both strands from the same individual) are removed. The E. coli mismatch repair enzyme selection facilitates the removal of most of the mismatch-containing heterohybrids (6 ,7 ), therefore, DNA fragments from all IBD regions are isolated on the basis of their ability to form extended mismatch-free heterohybrids (double-stranded DNA [dsDNA] molecules consisting of one strand from each of the individuals). These GMS-enriched mismatch-free heterohybrids are likely to include a disease gene locus inherited through a common ancestor.
        http://img.dxycdn.com/trademd/upload/asset/meeting/2014/02/13/B1392271718.jpg
        Fig. 1.  A schematic outline of the GMS process.

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序