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An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil

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An accelerated soil bait test can be used to determine whether a field harbours virus-carrying Spongospora subterranea . S. subterranea is the causal agent of powdery scab and also the only vector of potato mop top virus (PMTV). Real-time RT-PCR can detect PMTV RNA in the roots of bait plants after 2 weeks of growth in viruliferous soil. This test may be used to assess the risk of planting potato crops in a particular field.
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