Methods to Study the Role of Sirtuins in Genome Stability
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One of the most important roles of Sirtuins is to ensure the maintenance of genome integrity under stress conditions. In this chapter, we provide a methodology to study this role of Sirtuins at two different levels: detection of genomic instability (with the Neutral Comet Assay) and study of Sirtuin dynamics in chromatin under stress conditions (by isolating insoluble chromatin fractions).
