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Base:
| Bacto Heart Infusion Broth | 1.2% |
| Bacto Yeast Extract | 0.5% |
| Bacto Peptone | 0.5% |
| NaCl | 0.025% |
| Na-DNA | 0.002% |
pH is adjusted to 8 and autoclaved.
May be stored at room temperature for 4 weeks
ADD (to final conc.)
| K2HPO4 | 0.17% |
| Glucose | 1.0% |
| Thallium Acetate | 0.025% |
| Penicillin | 500u/ml |
| Horse Serum | 10% |
| Na-DNA | 0.0025% |
pH to 7.8
4% culture is added and grown statically at 37°C for 18-20 hours to A260
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