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        Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers

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        The methyl-combing technique combines the dynamic molecular combing method with the detection of DNA modifications. The assay allows the single-molecule analysis of epigenetic marks on regularly stretched DNA fibers, at the megabase scale with kilobase resolution. The protocol presented in this chapter is based on proof-of-principle experiments where the single-molecule detection of DNA methylation has been performed on unmethylated and in vitro methylated λ phage DNA.
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