Separation of Amino Acids by Capillary Electrophoresis with Light-Emitting Diode-Induced Fluorescence in the Presence of Electroosmotic Flow
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In this chapter, we describe a method to identify amino acids (AA) by capillary electrophoresis in conjunction with light-emitting
diode-induced fluorescence (LEDIF). First, amino acids labeled with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence
of cyanide are converted to highly fluorescent cyanobenz[f
]isoindole (CBI) derivatives. Next, they are separated by gel electrophoresis in the presence of EOF. In the process, the
CBI products were excited by a violet LED to produce green fluorescence. In addition to the optical setup of light-emitting
diode-induced fluorescence, the preparation of poly(ethylene) oxide for amino acid separation is also described in this chapter.
![[(1S)-1-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-(6-aminohexanoylamino)-3-phenylpropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]hexanoyl]amino]-4-bromobutyl]boronic acid，Moligand™，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/722/0483842343985733081.jpg!wh200)
