Separation of Amino Acids by Capillary Electrophoresis with Light-Emitting Diode-Induced Fluorescence in the Presence of Electroosmotic Flow
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In this chapter, we describe a method to identify amino acids (AA) by capillary electrophoresis in conjunction with light-emitting
diode-induced fluorescence (LEDIF). First, amino acids labeled with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence
of cyanide are converted to highly fluorescent cyanobenz[f
]isoindole (CBI) derivatives. Next, they are separated by gel electrophoresis in the presence of EOF. In the process, the
CBI products were excited by a violet LED to produce green fluorescence. In addition to the optical setup of light-emitting
diode-induced fluorescence, the preparation of poly(ethylene) oxide for amino acid separation is also described in this chapter.
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