Two-Dimensional Protein Analysis of Neural Stem Cells
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The current protocol describes in the first part the isolation and culture of neural stem and progenitor cells. In the second
part, the two-dimensional gel electrophoresis of neural stem and progenitor cells is explained, consisting of sample preparation,
first-dimensional isoelectric focusing, second-dimensional acrylamide gel electrophoresis, protein staining, and image digitalization.
The third part concentrates on the software analysis of two-dimensional gel images and the bioinformatical processing.
