• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Manipulation of Early Region 4

        互联网

        1007
        Early region 4 (E4; Fig. 1A ) occupies the right-hand 3000 bp of the human adenovirus genome. The sequences of E4 and E4 cDNAs indicate that E4 encodes seven polypeptides ( 14 ), most of which have been detected in infected cells. Analysis of E4 mutants has implicated E4 products in a variety of processes that occur in infected cells, including viral early and late gene expression, DNA replication, the shutoff of host-cell protein synthesis, transformation, and the ability to stimulate replication of adenovirus-associated virus (AAV). Specific E4 products have been implicated in many of those processes and, in some cases, information on the molecular mechanisms of E4 function is emerging. Current knowledge of the functions of E4 products is summarized below.
        Fig. 1  A ) The organization of adenovirus early region 4 E4 open reading frames (ORFs) are indicated by open boxes above a scale that indicates position in map units (0 to 100; 1 map unit is approx 360 bp) and nucleotide numbers. ORFs 1,2,3,4, and 6 are colinear with the viral DNA; ORFs 3/4 and 6/7 are the result of inframe splicing of the E4 mRNA precursor E4 is transcribed from right to left. ( B ) Reconstruction of an intact viral genome by ligation in vitro ( C ) Reconstruction of an intact viral genome by overlap recombination. ( D ) Construction of an E4 mutant by recombination between a plasmid and an E4 deletion mutant In C and D, the X indicates a potential homologous recombination event. In B, C, and D, the position of a hypothetical E4 mutation is noted by a V

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序