Enantiomeric Separations by HPLC Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview

The development of effective high efficiency enantiomeric separations is a tremendous success story (1 ). The separation of enantiomers is now accomplished by chiral chromatographic and electrophoretic methods, which includes gas chromatography (GC), high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), supercritical fluid chromatography (SFC), capillary electrochromatography (CEC), and capillary electrophoresis (CE). Enantioselective HPLC is the most widely used chromatographic method, both for analytical and preparative purposes, in most branches of science and technology including the pharmaceutical and environmental fields. The search for more effective and universal chiral stationary phases (CSPs) for HPLC is an ongoing and challenging topic for separation scientists.