Phage-Display Libraries
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Foreign proteins can be displayed on the surface of filamentous bacteriophage by fusing them to the phage minor coat protein
(Fig. 1
) Many proteins have been expressed in this way, including human growth factor (1
), alkaline phosphatase (2
), ricin (3
), pancreatic trypsin inhibitor (4
), and a DNA-binding protein (5
) Most importantly, the displayed proteins retain their biological activity
