T-Cell Receptor Purification
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The antigen receptor expressed on most T-lymphocytes (T-cell antigen receptor [TCR]) is a multisubunit complex consisting
of at least six different polypeptides (α, β, γ, δ, ɛ, and ζ), several of which are modified by addition of N
-linked oligosaccharide chains Oligosaccharide side chains on TCR glycoproteins undergo well-characterized processing events
within the endoplasmic reticulum (ER) and Golgi systems, including removal of mannose residues and subsequent addition of
galactose and sialic acid oligosaccharides. In the current chapter, a method is described for the isolation of immature and
mature TCR complexes from murine T-lymphocytes using lectin affinity chromatography This technique is rapid, sensitive, and
does not disrupt the integrity of assembled TCR complexes.
