• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells

        互联网

        457
        The surfaces of living cells contain a wide variety of molecular species that are characteristic of the type and physiological role of the cell. These surface molecules mediate cell-recognition events, receptorligand interactions and hormonal signaling events, surface homeostasis and transport regulation, attachment to surrounding structures, and a host of other physiologically vital functions. The types of surface molecules present include integral, as well as peripheral elements, and include proteins, carbohydrate moieties, and lipids. The number of each molecular species that serves a physiological function can vary from only a few hundred molecules per cell on the surface to many millions of molecules per cell. The unique nature of many of these molecules was recognized early in the development of immunologic techniques for cell identification, and this serves as the basis for many highly useful diagnostic tools in clinical medicine. The identification of surface antigenic molecules using antibodies can be performed by several methods, including immunofluorescence microscopy, enzyme and discrete marker microscopy, enzyme and radioactive labeling of mass cultures, and flow cytometry using fluorescence dyes. In this chapter, methods will be described that concentrate on the immunofluorescence microscopy approach to surface antigen identification in cultured cells (1 ,2 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序