• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center

        互联网

        359
        In recent decades, laboratories throughout the world generated several thousand mutant, transgenic, and wild-type zebrafish lines and more lines continue to be produced. At the same time, relatively little effort has been expended to develop reliable, high-throughput, standardized, long-term cryopreservation storage methods, even though laboratories and the research community as a whole struggle to maintain the large number of lines alive. Safe and reliable methods for maintaining these valuable genetic resources are vital for future biomedical research.
        Cryopreservation is the most efficient method for large-scale, long-term storage of important genetic materials. It extends the time offspring can be produced from individual fish, reduces the need to maintain live populations, and can prevent catastrophic loss of irreplaceable research lines. Cryopreservation is also the most cost-effective alternative for maintaining genetic resources because it reduces costs for animal and facility maintenance, personnel, and space. In addition, it provides novel opportunities to develop new types of research using large numbers of lines. For example, several genetic strategies, such as TILLING—or enhancer and gene trapping—depend on the use of cryopreservation to bypass generations of live organisms until a strain is revived for research.
        This chapter describes and discusses the current cryopreservation method used at the Zebrafish International Resource Center. This method is derived from the initial protocol developed for zebrafish over 20 years ago that has recently been refined.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序