Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens

Plant cell wall proteins play essential roles in many important biological processes, and yet there is still not a comprehensive catalogue of the cell wall proteome, or “secretome”. Here, we describe three procedures, including a yeast secretion trap (YST), Agrobacterium -mediated transient expression using a necrosis-inducing protein (NIP) and protein localization assay using a fluorescent protein, to identify and confirm the localization of cell wall proteins. The approaches are orthogonal and collectively provide a powerful suite of approaches to complement more commonly used strategies to isolate plant cell wall-associated proteins.