Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids
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A protocol for the establishment of in vitro shoot cultures of Catharanthus roseus is described. Shoots can be maintained for more than 1 yr without evidence of tissue vitrification, disaggregation, or callus formation. Vindoline was the main alkaloid accumulated, reaching values similar to those found in leaves from field-grown plants, after a long period of culture. An induction methodology to reduce such waiting time is also presented.
![Ethyl 3-(3,4-difluorobenzoyl)-1,1-dimethyl-1,2,3,6-tetrahydroazepino[4,5-b]indole-5-carboxylate，629662-23-3，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/630/0420372030125733081.jpg!wh200)
