下载molecular clonging 第三版（PDF）
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http://mededucation.bjmu.edu.cn/mc3/molecular%20clonging%203.pdf
Table of Contents：
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
Chapter 2: Bacteriophage and Its Vectors
Chapter 3: Working with Bacteriophage M13 Vectors
Chapter 4: Working with High-Capacity Vectors
Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
Chapter 10: Working with Synthetic Oligonucleotide Probes
Chapter 11: Preparation of cDNA Libraries and Gene Identification
Chapter 12: DNA Sequencing
Chapter 13: Mutagenesis
Chapter 14: Screening Expression Libraries
Chapter 15: Expression of Cloned Genes in Escherichia coli
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
Chapter 18: Protein Interaction Technologies
Table of Contents：
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
Chapter 2: Bacteriophage and Its Vectors
Chapter 3: Working with Bacteriophage M13 Vectors
Chapter 4: Working with High-Capacity Vectors
Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
Chapter 10: Working with Synthetic Oligonucleotide Probes
Chapter 11: Preparation of cDNA Libraries and Gene Identification
Chapter 12: DNA Sequencing
Chapter 13: Mutagenesis
Chapter 14: Screening Expression Libraries
Chapter 15: Expression of Cloned Genes in Escherichia coli
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
Chapter 18: Protein Interaction Technologies
