X-Ray Diffraction to Determine the Thickness of Raft and Nonraft Bilayers
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Low-angle X-ray diffraction is a powerful method to analyze the structure of membrane bilayers. Specifically, the technique
can be used to determine accurately the thickness of fully hydrated bilayers. Herein details are presented showing how this
technique can measure the difference in thickness of bilayers in detergent-resistant membranes and detergent-soluble membranes
extracted from model systems known to contain both raft and nonraft domains. The observed thickness difference may be critical
in the sorting of transmembrane proteins between raft and nonraft bilayers.
