Sequencing Using the Du Pont Genesis™ 2000 DNA Analysis System

The Du Pont Genesis^™ 2000 DNA Analysis System consists of an instrument, reagents capable of labeling DNA with novel fluorescent dideoxynucleotides (1 ), and a Macintosh® II computer to operate the instrument and perform all subsequent data analysis The instrument houses a vertical electrophoresis apparatus (similar to a standard manual sequencing setup) with associated buffer reservoirs and high voltage power supply, together with a fluorescence detection system An argon laser sweeps across the 12-lane sequencing gel and excites fluorescently labeled DNA fragments as they electrophorese past a fixed window toward the bottom of the gel, and the emitted fluorescence is detected simultaneously by two photomultiplier tubes with differing filters (Fig 1 ), and a Macintosh® II computer to operate the instrument and perform all subsequent data analysis. The instrument houses a vertical electrophoresis apparatus (similar to a standard manual sequencing setup) with associated buffer reservoirs and high voltage power supply, together with a fluorescence detection system. An argon laser sweeps across the 12-lane sequencing gel and excites fluorescently labeled DNA fragments as they electrophorese past a fixed window toward the bottom of the gel, and the emitted fluorescence is detected simultaneously by two photomultiplier tubes with differing filters (Fig. 1 )

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