Electrophoresis of Sequence Reaction Samples
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The underlying principle of both main DNA sequencing methods by Sanger (1 ) and Maxam and Gilbert (2 ), is the ability to fractionate and resolve long, single-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (3 ).
