• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes

        互联网

        666
        Ion-selective microelectrodes can be used to measure intracellular ion concentrations. The use of multi-barrelled electrodes enables the identification of the cellular compartment. For example, the inclusion of a pH-selective electrode enables the cytoplasm and vacuole to be distinguished. The ion-selective barrels of microelectrodes are filled with a sensor cocktail containing several different components.
        An ion-selective molecule, sensor or exchanger.
        Membrane solvent or plasticizer.
        Additives, e.g., lipophilic cation/anion.
        Membrane matrix to solidify the ion-selective membrane; essential for measurements in plant cells with a cell wall and turgor.
        For many ions, the ready-made membrane cocktail can be purchased, but the individual chemical components can be bought from suppliers and mixing the cocktail oneself is cheaper. For commercially available liquid membrane cocktails, the membrane matrix is not normally included. A matrix is needed if the microelectrodes are to be used in plants because cell turgor will displace a liquid membrane from the electrode tip, thereby changing or eliminating the sensitivity to the measuring ion. The matrix used is usually a high molecular weight poly(vinyl chloride), but can include other polymers, such as nitrocellulose for additional strength.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序