Improved Shoot Regeneration from Root Explants Using an Abscisic Acid-Containing Medium

Arabidopsis thaliana is an excellent experimental system to study various aspects of plant biology. An efficient regeneration protocol of the plant is important to produce in vitro fertile plants, as well as a fundamental step to study the cellular, physiological, and molecular mechanisms of plant development. Here, we describe an efficient and fast regeneration system from root explants of A. thaliana ecotypes Columbia-0 and Landsberg erecta. Culture conditions and media compositions were optimized for the development of higher number of plantlets. We demonstrate that the combination of the plant regulators abscisic acid and cytokinin in the shoot induction medium increases the formation of shoots as well as reduces the proliferation of abnormal tissues.