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也称插入因子，是指能插入到细胞染色体和质粒上去的特殊的 DNA短的顺序。插入顺序可分为 I、Ⅱ、Ⅲ（ IS1， IS2， IS3）。 IS1约由 800对核苷酸、 IS2约由 1400对核苷酸构成的。从大肠杆菌的染色体和 F因子，λ噬菌体等上面可见到同一插入顺序。这些顺序一旦插入基因，不仅该基因失去了功能，并且也常对邻接的基因群的功能产生极性效应。插入顺序的来源还不清楚，它们的分布相当广泛，现在如同对基因的重组，质粒插入寄主染色体，基因的缺失等过程一样，正在研究有关插入顺序间的重组。
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