• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases

        互联网

        600
        Nucleases catalyze the cleavage of phosphodiester bonds in nucleic acids. They range in size from divalent cations, such as Pb2+ , which specifically cleaves tRNAPhe from yeast (1 ), and Mg2+ , which specifically acts on squid tRNALys (2 ) to divalent cations complexed to chelating agents, for example Fe2+ -bleomycin (3 ) and Cu2+ -phenanthroline (4 ) or imidazole (5 ), to complex nucleic acids and proteins, such as ribozymes (6 ,7 ) or the ribonucleoprotein RNaseP (8 ,9 ). Protein nucleases are involved in a variety of important cellular functions, such as DNA restriction, DNA repair and recombination, proofreading of DNA replication, DNA cleavage during programmed cell death and RNA processing, maturation and editing (10 ,11 ). Nucleases may be extremely specific; for example, homing endonucleases, or nonspecific. Examples of the latter include nucleases found in the gastrointestinal tract of higher vertebrates and those secreted into the medium by various microorganisms.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序