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        Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells

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        Adhesion of metastatic cancer cells to the vascular endothelium of the target organs and their subsequent transendothelial migration is one of the critical, yet poorly understood, steps of the metastatic cascade. Conventionally, the mechanisms of this complex process have been studied using static adhesion systems or flow assay systems. Static assay systems are easy to set up and perform but do not mimic the physiological conditions of blood flow. Flow assays closely mimic physiological conditions of flow but are time consuming and require specialist equipment. In this chapter we describe the rocking adhesion system which incorporates the key advantages of both the static and flow assay systems and not only is easy to set up and perform but also mimics conditions of blood flow.
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