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Preparation of cell lysates from yeast by enzymatic digestion of the cell wall
Materials
Chemicals
| Lyticase [or Zymolase 100T (ICN, USA)] |
| Brij 58 |
| <center> <font>50 mM Tris-HCl pH 8.0 </font> <p> <font>10% sorbitol</font></p> </center> |
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