• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Stepwise Differentiation from Nave State Pluripotent Stem Cells to Functional Primordial Germ Cells Through an Epiblast-Like State

        互联网

        580
        A group of pluripotent cells appearing during mammalian embryogenesis is the source for all the cell lineages that compose the embryo proper. In mice, pluripotent cells are first established in the inner cell mass (ICM) of the preimplantation blastocyst. After implantation, the ICM soon transforms into a cup-shaped epithelium, called the postimplantation epiblast. The two types of pluripotent cells, the ICM and postimplantation epiblast cells, are distinct, based on the differences in their gene expression profiles, epigenetic status, and differentiation capacity. During gastrulation, some of the postimplantation epiblast cells adjacent to the extraembryonic ectoderm are specified as primordial germ cells (PGCs), precursors of the germ cell lineage, in response to bone morphogenetic protein 4 (BMP4). Recently, we succeeded in reconstituting epiblast differentiation and PGC specification in vitro using pluripotent stem cells. Here, we describe the culture method of a stepwise differentiation from pluripotent stem cells to functional PGCs.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序