• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Signal Amplification for DNA and mRNA

        互联网

        629
        In situ hybridization (ISH) permits the localization of specific unique or repeated DNA and RNA sequences at the level of individual cells (1 -4 ). It has significantly advanced the study of gene structure and expression, and, in addition to morphological identification of cell types involved, ISH also allows some quantification of observations, e.g., with respect to tumor burden or viral load. Despite its high degree of detection specificity, the technique still does not allow the routine detection of DNA sequences less than 5 kb in size, and in the case of tissue sections the detection sensitivity is even more limited. The threshold levels for mRNA detection are more difficult to determine, with the reported sensitivity limits of 1–20 copies of mRNA per cell being achieved only in the most sensitive protocols (5 ,6 ,6a ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序