A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry

Recognition of pathogenic yeasts by host cells is based on components of the yeast cell wall, which are considered part of its virulence attributes. Cell wall glycans play an important role in the continuous interchange that regulates the balance between saprophytism and parasitism and between resistance and infection. Flow cytometry is a useful method for probing surface yeast glycans in order to compare their expression depending on strains and growth conditions. By using different monoclonal or polyclonal antibodies, levels of β- and α-linked mannosides as well as β-glucans can be successfully evaluated by flow cytometry methods. The cytometric method we describe here represents a useful tool to investigate to what extent yeasts are able to regulate their glycan surface expression and therefore modify their virulence properties.