细菌检测
互联网
2287
-
Gram Staining (+\-) (William H. Heidcamp)
-
Gram-Staining Procedure (MEDIC, U of Texas)
Very nice and detailed method description for Gram staining
-
Acid-Fast Stain (Putt's Method)
To detect the presence of acid-fast mycobacteria in tissue sections -
Smear Preparation (MEDIC, U of Texas)
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
-
Alkaline Phosphatase and Phospholipase C Assay (Hancock Lab) (Accessible only by Internet Explorer)
-
Bacteria Agglutination Assay (Hancock Lab) (Accessible only by Internet Explorer)
Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120o C for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cells grown to mid log phase, then centrifuged and resuspended in saline.
-
Carbohydrate Assay (Hancock Lab) (Accessible only by Internet Explorer)
This assay is used to determine the relative amounts of LPS CHO present in a given strain and can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars.
-
Dintrophenylation Assay for Polymixin B (Hancock Lab) (Accessible only by Internet Explorer)
-
Endotoxin Assay (Hancock Lab) (Accessible only by Internet Explorer)
This assay measures the shift in the absorbance maximum of the dye due to binding to endotoxin
-
Indirect Immunfluoresence (Hancock Lab) (Accessible only by Internet Explorer)
-
Liposome Swelling Assay (Hancock Lab) (Accessible only by Internet Explorer)
Also including method for liposome Isolation and liposome preparatio n
-
Preparation of E Coli Protein for SDS-PAGE (Hahn Lab)
Simplified method for analyzing E coli protein
