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        【求助】求助翻译:colony purify是什么意思啊

        丁香园论坛

        1247
        Shocked cells were added to 1-ml SOC, incubated 1 h at 37°C, and then one-half was spread onto agar to select CmR or KmR transformants. If none grew within 24 h, the remainder was spread after standing overnight at room temperature. After primary selection, mutants were maintained on medium without an antibiotic. They were colony-purified once nonselectively at 37°C and then tested for ampicillin
        sensitivity to test for loss of the helper plasmid. If it was not
        lost, then a few were colony-purified once at 43°C and similarly tested.

        colony purify是指丢失helper plasmid吗?“tested for ampicillin sensitivity to test for loss of the helper plasmid”具体应该怎么做呢??
        1\colony purify是指丢失helper plasmid吗
        ===背景不清晰,不好回答。从字面意思理解,colony purify是指挑取来自primary selection的单个克隆,进一步扩增。

        2\tested for ampicillin sensitivity to test for loss of the helper plasmid
        没有语境,不好判断。可以写信给作者询问。
        freecell wrote:
        1\colony purify是指丢失helper plasmid吗
        ===背景不清晰,不好回答。从字面意思理解,colony purify是指挑取来自primary selection的单个克隆,进一步扩增。

        wrote:
        2\tested for ampicillin sensitivity to test for loss of the helper plasmid
        没有语境,不好判断。可以写信给作者询问。


        第2句话的意思,应该就是检测安芐敏感性,用于测试是否丢失了质粒(质粒携带安芐抗性),至于方法,直接挑菌,放液体培养基摇半小时,再到含安芐的培养基上划平板,看是否能生长即可。

        本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴

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        【求助】求助翻译:colony purify是什么意思啊

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