• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Gene Targeting Vector Design for Embryonic Stem Cell Modifications

        互联网

        445
        The use of genetically engineered mice to understand gene function is widespread. Changes to the mouse genome can be introduced with gene targeting vectors or with transgenes. Targeting vectors are usually used to ablate gene expression while transgenes are designed to express proteins that are normally absent from the organism. For example, gene targeting in mouse embryonic stem cells can be used to generate a mutant mouse model that fails to express a physiologically important protein. Transgenes that express the missing protein or a substitute for the missing protein can be used to assess possible gene therapies for the mutant mouse. Both gene targeting and transgene approaches can be used to study regulatory elements that control gene function. Putative control elements can be added to or removed from the chromosome with targeting vectors. Transgenes carrying long DNA sequences that include different combinations of potential control elements can be introduced into the genome to assess their effects on gene expression. The exploration of how genes interact to control development, homeostasis, and pathophysiological conditions can be dissected by introducing carefully designed genetic constructs into model organisms.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序