Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC/MS/MS)
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A detailed protocol using liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) is described for the determination of the total homocysteine (HCY) in plasma. Sample preparation is simple and relies on the complete reduction of any homocystine ((HCY)2 ) or protein-bound homocysteine, followed by the plasma protein precipitation. Any factor influencing the measurement is addressed. The protocol is robust and suitable for large-scale routine determinations.
