Lentiviral Vector Transduction of Dendritic Cells for Novel Vaccine Strategies

Dendritic cells (DCs) are key antigen-presenting cells that induce primary and memory immune response. Patients with chronic infections or cancer often display DC dysfunctions. Modification of DCs or DC progenitors in vitro may overcome the problems with defective DCs in vivo. Lentiviral vector is highly efficient in transducing hematopoietic cells including DCs. Examples of lentiviral modification of DCs with immune modulatory genes and analysis of antigen-specific T cells to demonstrate enhanced immune effector functions of DCs will be introduced.