Phosphoinositidase C Activation Assay II: Simple Analysis of Recovered Cellular Phosphoinositides and Phosphoinositols

In Chapter 1 , procedures for cell labeling with [³ H]inositol, stimulation with agonists, and extraction of the phosphoinositols and phosphoinositides are described. In this chapter, simple low-resolution chromatography techniques capable of separating phosphoinositols and phosphoinositides into their general classes using inexpensive apparatus are described in detail. For higher resolution chromatographic techniques capable of separating isomeric forms, see Chapters 3 and 4 .