细胞组分和细胞器――细胞骨架
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3113
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Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab)
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Recycling Tubulin (Mitchison Lab)
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Labeling Tubulin and Quantifying Labeling Stoichiometry (Mitchison Lab)
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Tubulin Polymerization with GTP/GMPCPP/Taxol (Mitchison Lab)
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Preparation of Segmented and Polarity Marked Microtubules (Mitchison Lab)
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Microtubule Spindowns for Visual Analysis (Mitchison Lab)
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Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC (Mitchison Lab)
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Negative Stain Electron Microscopy of Microtubules (Mitchison Lab)
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Microtubule Pelleting (Mitchison Lab)
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Actin - Chromatography Purification (The Cell Biology and Cytoskeleton Group, HMS)
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Actin - Labeling with Pyrene Iodoacetamide (The Cell Biology and Cytoskeleton Group, HMS)
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Actin Binding Assay with Rhodamine-Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
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Actin Extraction from Acetone Powder (The Cell Biology and Cytoskeleton Group, HMS)
- Actin Preparation - Acetone Powder Method (The Cell Biology and Cytoskeleton Group, HMS)
- Plasma Gelsolin Preparation (The Cell Biology and Cytoskeleton Group, HMS)
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Some Buffers for Actin Work (The Cell Biology and Cytoskeleton Group, HMS)
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Fixing and Immunostaining Cells (The Cell Biology and Cytoskeleton Group, HMS)
- Fixing and Staining Cells for Actin with Fluorescently-Labeled Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
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"Cells on Gels" (The Cell Biology and Cytoskeleton Group, HMS)
this protocol describes method for culture cell on a thin polyacrylamide-based, collagen-coated flexible substrate. By maintaining a constant total concentration of acrylamide while usr/localying the concentration of bis-acrylamide, it's able to obtain a series of chemically identical substrates with a wide range of flexibility. By using imaging techniques, cells' response to differences in substrate flexibility can be detected.
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Immunofluorescent Localization of Tubulin (William H. Heidcamp)
For cultured cell
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Isolation of Microtubules (Bovine Brain) (William H. Heidcamp)
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Viscosity & Polymerization of Microtubules (William H. Heidcamp)
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TEM Visualization of Microtubules (William H. Heidcamp)
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SDS Gel Electrophoresis of Tubulin\MAPs (William H. Heidcamp)
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Isolation of Actin and Myosin Filaments (William H. Heidcamp)
- Yeast Actin Capture Assay (D. Amberg)
