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        Phenotyping of Zebrafish Homebase Behaviors in Novelty-Based Tests

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        Various novelty-based assays used to quantify zebrafish (Danio rerio ) behavior show a striking similarity to behavioral responses in rodents. Exposed to the open field test, zebrafish establish overt homebases demonstrating clear preference for a particular area of the tank. This behavior aims to establish a “safe zone” that zebrafish can familiarize themselves with and feel secure in, and is similar to homebase behaviors of various laboratory rodent species. Here we outline a simple protocol for homebase phenotyping in zebrafish.
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