• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Directing Epidermal Fate Selection by a Novel Co-Culture System

        互联网

        533
        We describe an improved culturing protocol to direct embryonic stem (ES) cell differentiation along the epidermal lineage in vitro. Pluripotent ES cells co-cultured with mitomycin C-treated mature epidermal cells at low density resulted in colonies that undergo differentiation to keratin 14 (K14)-positive cells within 3 d in culture. This new protocol suggests that the commitment and differentiation of undifferentiated ES cells can be efficiently directed by mature epidermal cell-derived factors. In addition, it suggests that this condition may be responsible for the maintenance and expansion of putative epidermal stem cells in vitro. Our advanced culturing regimen allows for the analysis of colony formation from a single cell to determine the factors controlling the differentiation capacities of very early progenitor cells in culture.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序