Development of Trainable Deoxyribozyme-Based Game Playing Automaton

Molecular automata are self-operating machines serially exchanging information with their environment while changing their configurations. Molecular protoautomata are devices trained in a series of sessions with an operator to become molecular automata. Reconfigurable deoxyribozyme-based logic gates can be used to build multipurpose protoautomata, reprogrammable devices that go beyond single-purpose “hard-wired” molecular automata. Molecular array of YES and AND gates (MAYA)-III is such a protoautomaton that can be taught by example to play a game. Training of MAYA-III is a process that does not require the operator to be familiar with the underlying molecular programming. Herein we provide the instructions to construct this protoautomaton, with particular focus on the optimization of computing components.