Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids
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This procedure yields thin flakes of freeze-dried material from tissue culture cells. Up to 0.5 g of wet cells can be processed at one time. Dried cells, stored indefinitely at −20�C, have full lactate dehydrogenase activity (1 ) and DNA polymerase activity (2 ). The dried cells stored for a few days at room temperature also have apparently undegraded nucleic acids (3 ).
