Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids
互联网
345
This procedure yields thin flakes of freeze-dried material from tissue culture cells. Up to 0.5 g of wet cells can be processed at one time. Dried cells, stored indefinitely at −20�C, have full lactate dehydrogenase activity (1 ) and DNA polymerase activity (2 ). The dried cells stored for a few days at room temperature also have apparently undegraded nucleic acids (3 ).
