EPON resin mixture for transmission electron microscopy
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EPON resin mixture for transmission electron microscopy
For Epon WPE 153:| ~120 ml | ~60 ml | ~30 ml | |||
|---|---|---|---|---|---|
| Mix A: | Embed 812 | 44 ml | 22.1 ml | 11.1 ml | |
| DDSA | 67 ml | 33.3 ml | 16.7 ml | ||
| Mix B: | Embed 812 | 67 ml | 33.3 ml | 16.7 ml | |
| NMA | 56 ml | 27.8 ml | 13.9 ml |
Prepare mixture A and mixture B in 30 - 120 ml batches. Mix well and store in glass scintillation vials or some other container that can be capped tightly. Store at -20 degrees C. The mixtures can be stored for years.
For use:
- Be certain to completely warm the vials before opening them to prevent water from mixing with the plastic.
- Mix equal volumes of A and B together.
- Then add 0.1 ml of DMP-30 for each 5 ml of plastic.
- Mix well and store no longer than a day at room temp. I usually put the remainder of the plastic in a tube or vial, cap and wrap the cap with parafilm, and put it at 4 degrees C overnight.
