Microdeletion screening

Microdeletion screening

<center> <table> <tbody> <tr> <td> <p>  </p> <hr /> <font color="#af0000"><font>One application of multiplex PCR is microdeletion screening. This can be applied to the X and Y chromosomes (male genomic DNA) or to hybrid cell lines (rodent-human) containing one copy only of a human chromosome of interest.</font> </font> <p>  </p> <p> <font color="#af0000"><font>Figures below show a few examples of multiplex PCR screening reactions for microdeletions on human chromosome Y.</font> </font></p> <ul> <li> <font color="#af0000"><font><b>Fig. 43 </b> shows microdeletion screening reactions with multiplex mixtures A and B</font> </font></li> <li> <font color="#af0000"><font><b>Fig. 44</b> shows microdeletion screening reactions with mixture D (gel separation in 4 hours at high voltage)</font> </font></li> <li> <font color="#af0000"><font><b>Fig. 45</b> shows microdeletion screening reactions with mixture D but in conditions in which gel separation was performed overnight (16 hours) at low voltage. PCR products are visible but more diffused. See also page 15.</font> </font></li> </ul> </td> </tr> <tr> <td> <p>  </p> <hr /> <p>  </p> </td> </tr> <tr> <td> <p> <font color="#5247f1"><b>Fig. 43.</b> Y-chromosome microdeletion screening reactions of 12 male genomic DNA samples (yellow) using multiplex mixture B (5 loci) and of 11 DNA samples (green) using multiplex mixture A (7 loci). DNA samples 1, 2, 9, 10 and 12 (yellow) and 1 and 2 (green) show deletion of some loci tested (lack of amplification products). </font> <font color="#5247f1"> </font></p> <hr /> <p>  </p> </td> </tr> <tr> <td> <p> <font> </font></p> </td> <td> <p> <font color="#5247f1"><b>Fig. 44. </b> Y-chromosome microdeletion screening reactions of 16 male genomic DNA samples (yellow) using multiplex mixture D (5 loci). DNA samples 10, 11, 12, and 13 show deletion of some loci tested. Gel separation was done at high voltage, in about 3-4 hours. See also Fig. 45 for comparisons.</font></p> </td> </tr> <tr> <td> <p> <font> </font></p> </td> <td> <p> <font color="#5247f1"><b>Fig. 45.</b> Y chromosome deletion screening using esentially the same DNA samples and primer mixture D as in Fig. 44 above. Only the order of the samples on the gel was somewhat changed. Gel separation was done overnigtht (16 hours) at low voltage. Products appear much more diffuse but result interpretation can be easily done. </font></p> </td> </tr> </tbody> </table> </center>